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Tuesday, October 11, 2022

When the covid injection mRNA does indeed reverse transcriptase into your DNA


HERE IS THE PAPER:
LINE-1-Mediated Reverse Transcription of Vaccine mRNA Researchers in Sweden have conducted an in vitro study on a human hepatic carcinoma cell line (Huh7 cells) exposed to the Pfizer BioNtech BNT162b2 vaccine, specifically examining the question of whether these cells have the capability of converting the mRNA in the vaccine into DNA [19]. 
The authors found that the cells readily and spontaneously took up the mRNA nanoparticles and responded to exposure by upregulating LINE-1. An immunohistochemistry assay revealed that LINE-1 levels were increased in the nucleus in response to the mRNA nanoparticles. 
Alarmingly, they verified that a 444 base pair reporter region (amplicon) of mRNA was readily reverse transcribed intracellularly into DNA as soon as 6 hours following exposure. 
Tracer studies have shown that the mRNAs in the vaccines enter the lymph system and are eventually taken up by cells in multiple organs, with the liver being second only to the spleen in the concentration detected [20]. It was suggested by Aldén et al. that the liver cells could be exposing spike protein on their surface and in this way inducing an 6 autoimmune attack on the cells by antibodies [19]. 
This might explain several observed cases of autoimmune hepatitis in response to the vaccine [21-23]. The mRNA in the vaccines has been engineered to have a long poly(A) tail, which helps both to facilitate translation into protein and increase survival time of the mRNA. 
However, the presence of a large number of mRNA molecules with long poly(A) tails likely increases expression of poly(A) binding protein (PABP), to serve the needs of these mRNA molecules. PABP has been found to be essential for efficient LINE-1 retrotransposition, and knockdown of PABP greatly decreases LINE-1 activity [24]. LINE-1 proliferation involves a complex life cycle beginning with RNA polymerase II (Pol II) transcription of its mRNA. 
The mRNA is translated into its two ORFs in the cytoplasm. The ORFs form a ribonucleoprotein (RNP) particle which then transfers to the nucleus for translation of the RNA into DNA and integration into the genome. It is hypothesized that PABP acts as an escort protein that can shuttle the RNP to the nucleus [25]. Thus, the mechanism by which the mRNA in the vaccines increases LINE-1 activity could be through upregulation of PABP.

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